Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: Apparent inhibition of receptor recycling
Identifieur interne : 003839 ( Main/Exploration ); précédent : 003838; suivant : 003840Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: Apparent inhibition of receptor recycling
Auteurs : Christopher Tietze [États-Unis] ; Paul Schlesinger [États-Unis] ; Philip Stahl [États-Unis]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1980.
English descriptors
- Teeft :
- Ammonium, Ammonium chloride, Biol, Biophysical, Biophysical research communications, Brief incubation, Cell biol, Cell surface, Cell surface receptors, Chloroquine, Control cells, Degradation, Fetal calf serum, Internalization, Ligand, Ligand uptake, Lysosomal, Lysosomal enzymes, Macrophage, Receptor, Receptor recycling, Second incubation, Uptake.
Abstract
Summary: 125I-Mannose-BSA is taken up by macrophages by receptor-mediated pinocytosis. Previous studies indicated that uptake is rapid and proceeds linearly with time in the absence of protein synthesis suggesting that receptors are conserved and recycled. Chloroquine and NH4+ inhibit uptake of 125I-Mannose-BSA and its subsequent digestion by macrophages. Inhibition of uptake is inhibitor-concentration and time dependent. Both binding of ligand (4°) and internalization of pre-bound ligand (4°) following warm-up are unaffected by the inhibitors. However, brief incubation of cells at 37° with inhibitor in the absence of ligand results in a substantial reduction in cell surface binding sites. Our interpretation of these results is that chloroquine and NH4+ inhibit receptor recycling and thereby inhibit ligand uptake.
Url:
DOI: 10.1016/S0006-291X(80)80237-3
Affiliations:
- États-Unis
- Missouri (État)
- Saint-Louis (Missouri)
- École de médecine (Université Washington de Saint-Louis)
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000763
- to stream Istex, to step Curation: 000763
- to stream Istex, to step Checkpoint: 002596
- to stream Main, to step Merge: 003918
- to stream Main, to step Curation: 003839
Le document en format XML
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incubation</term><term>Uptake</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: 125I-Mannose-BSA is taken up by macrophages by receptor-mediated pinocytosis. 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